2 edition of regulation of gene expression during Brassica napus embryogenesis. found in the catalog.
regulation of gene expression during Brassica napus embryogenesis.
James S. Keddie
Thesis (Ph.D.), University of East Anglia, School of Biological Sciences, 1993.
Many previous studies have provided evidence for genome changes in polyploids, but there are little data on the overall population dynamics of genome change and whether it causes phenotypic variability. We analyzed genetic, epigenetic, gene expression, and phenotypic changes in ∼50 resynthesized Brassica napus lines independently derived by hybridizing double haploids of Brassica . Wilf Keller's 26 research works with 1, citations and 1, reads, including: Perturbing the metabolic dynamics of myo-inositol in developing Brassica napus seeds through in vivo methylation. Background Studies in resynthesized Brassica napus allopolyploids indicate that homoeologous chromosome exchanges in advanced generations (S5∶6) alter gene expression through the loss and doubling of homoeologous genes within the rearrangements. Rearrangements may also indirectly affect global gene expression if homoeologous copies of gene .
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To gain insights into the genetic programmes that operate during embryo development in Brassica napus L. (canola), an important oilseed crop, we performed gene and protein expression Cited by: 5.
Seed development is controlled by a complex network of spatially and temporally expressed genes. We previously reported that carotenoid accumulation in Brassica napus seeds was developmentally regulated, with the highest levels detected at 35–40 days post-anthesis (DPA). To investigate accompanying changes in gene expression, we conducted a microarray study during Cited by: Using a system of secondary embryogenesis in Brassica napus cv.
Primor, we have attempted to address this problem by analysing the level of napin storage protein transcripts and their regulation Cited by: 7. In order to study Brassica napus fatty acid (FA) metabolism and relevant regulatory networks, a systematic identification of fatty acid (FA) biosynthesis-related genes was conducted.
Following gene identification, gene expression profiles during B. napus Cited by: This book describes how the genome sequence contributes to our understanding of allopolyploidisation and the genome evolution, genetic diversity, complex trait regulation and knowledge-based breeding of this important crop.
Gene Loss, Expression Divergence, and Epigenetic Regulation in Brassica napus. Abstract. Isolated microspores from selected cultivars of Brassica napus readily form embryos in culture after mild heat stress treatments (32°C for 1–3 days).
Transcript profiling methods were used to identify differentially-expressed genes as well as shifts in metabolism during the early stages of microspore embryogen-esis. Regulation of gene expression at the transcript level seems to be responsible for changes of BnSGT1 and BnSCT activities during seed and seedling development of B.
napus. Together with sinapine. Studies on resynthesized Brassica allotetraploids (specifically on synthetic oilseed rape, B. napus) have highlighted the high structural plasticity of the newly formed allopolyploid genome as well as highlighting numerous expression. Gene expression. Real-time PCR analysis indicated increased amount of dehydrin gene expression during cold stress in tolerant canola genotype.
It seems that all dehydrin groups were expressed in cold stressed B. napus. About Brassica napus. Oilseed rape (Brassica napus) regulation of gene expression during Brassica napus embryogenesis.
book formed ~7, years ago by hybridisation between B. rapa and B. oleracea, followed by chromosome doubling, a process known as er with more ancient polyploidsations, this conferred an aggregate fold genome multiplication since the origin of angiosperms and high gene.
AGL15 was initially identified as a differentially expressed gene that preferentially accumulates in Brassica napus embryos structure is a critical component of the epigenetic regulation of gene expression in both plants and for the analysis of gene expression during embryogenesis.
Comparative Transcriptome Analysis in Oilseed Rape (Brassica napus) Reveals Distinct Gene Expression Details between Nitrate and Ammonium Nutrition by Weijie Tang 1,2, Xin He 1,3.
DeLisle AJ, Crouch ML () Seed storage protein transcription and mRNA levels in Brassica napus during development and Perez-Grau L () Regulation of gene expression during plant embryogenesis. Cell – PubMed Hatzopoulos P.
() Developmental and Abscisic Acid Regulation of Gene Expression during Embryogenesis. Abstract. ABA has been shown to be involved in the control of many events during embryogenesis and seed development (Shiver and Mundy, ).
Many of the responses stimulated by ABA are also. About 18% of all EST-matched A. thaliana genes were matched by tags in antisense orientation, suggesting an involvement of poly A+ containing antisense RNAs in regulation of gene expression during B.
napus. Identification of gene expression profile during fertilization in Brassica campestris subsp. chinensis. Jingjing Jiang, a Jianxia Jiang, a Lin Qiu, a Ying Miao, b Lina Yao, a Jiashu Cao a.
a Laboratory of. Ectopic expression of the BBM gene, originally identified in Brassica napus, has diverse functions in plant cell proliferation, growth and development without exogenous growth regulators. We analyzed DNA sequences that regulate the expression of an isocitrate lyase gene from Brassica napus L.
during late embryogenesis and during postgerminative growth to determine whether glyoxysomal function is induced by a common mechanism at different developmental stages.
[beta]-Glucuronidase constructs were used both in transient expression assays in B. napus. Introduction. Oilseed rape (Brassica napus), an allotetraploid (4×) species, is the second-leading crop source of plant oil worldwide (Liu et al.,Smit et al., ).Increasing seed oil content (SOC) is the primary goal of.
Identifying genetic variation that increases crop yields is a primary objective in plant breeding. We used association analyses of oilseed rape/canola (Brassica napus) accessions to identify genetic variation that influences seed size, lipid content, and final crop yield. Variation in the promoter region of the HECT E3 ligase gene.
Crouch ML. Regulation of gene expression during seed development in flowering plants. Dev Biol (N Y ) ; – Fernandez DE, Turner FR, Crouch ML. In situ localization of storage protein mRNAs in developing meristems of Brassica napus.
Quatrano, R. S., Regulation of gene expression by abscisic acid during angio-sperm embryo development. In: Oxford Surveys of Plant Molecular and Cell Biology.
Vol.3, pp. – in the present study, dynamics of PME expression and degrees of pectin esterification have been analysed during microspore embryogenesis and compared with the gametophytic development, in Brassica napus.
A multidisciplinary approach has been adopted including BnPME gene expression. We examined the cytological profiles of oil body development and variation during the embryogenesis of B. napus cv.
Topas. The oil bodies were detected at the heart stage (Figure 2E–H), while oil. Introduction. Genomic imprinting is an epigenetic regulatory phenomenon in which there is allele-biased expression of certain genes depending on the parent of origin.
1 Imprinted gene expression plays a role in fetal growth regulation. We analyzed DNA sequences that regulate the expression of an isocitrate lyase gene from Brassica napus L. during late embryogenesis and during postgerminative growth to determine whether.
Phosphorus (P) is an essential macronutrient required for plant growth and development. The involvement of cytokinin response factors (CRFs) in phosphate (Pi) homeostasis and lateral root (LR) initiation in Arabidopsis has been revealed.
However, little is known in oil crops. Here, we performed genome-wide dissection of the CRF family in Brassica napus. A wide variation of morphological traits exists in Brassica rapa L. and Brassica oleracea L., and cultivated vegetable varieties of these species are consumed worldwide.
Flowering time is an. The present work has analysed the dynamics of DNA methylation patterns in relation to nuclear architecture and the expression of the DNA methyltransferase 1 gene MET1a during pollen development and pollen reprogramming to embryogenesis, in Brassica napus L.
The results show an epigenetic reprogramming after microspore induction to embryogenesis. Plant Cell Rep. Jul;34(7) doi: /s Epub Feb Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus.
Background. Serial analysis of gene expression (LongSAGE) was applied for gene expression profiling in seeds of oilseed rape (Brassica napus ssp. napus).The usefulness of this technique for detailed expression profiling in a non-model organism was demonstrated for the highly complex, neither fully sequenced nor annotated genome of B.
napus by applying a tag-to-gene. The regulation of gene expression during Brassica napus embryogenesis. Author: Keddie, James S. ISNI: Awarding Body: University of East Anglia Current Institution: University.
Goldberg RB, Barker SJ, Perez-Grau L. Regulation of gene expression during plant embryogenesis. Cell. Jan 27; 56 (2)– Guerche P, Tire C, De Sa FG, De Clercq A, Van Montagu M, Krebbers E. Differential Expression of the Arabidopsis 2S Albumin Genes and the Effect of Increasing Gene.
Transcriptome analysis of maize embryogenic callus and somatic embryos reveals associated genes reprogramming, hormone signaling pathways and transcriptional regulation involved in somatic embryogenesis.
An efficient and largely genotype-independent transformation method for Brassica napus and Brassica oleracea was established based on neo or bar as selectable marker genes. Hypocotyl explants of Brassica napus and Brassica.
Here, we report on the Brassica napus DOF gene, which is homologous to Arabidopsis Cycling DOF Factor1 and functions as a transcriptional regulator with regard to fl DOI: /s Somatic embryogenesis (SE) that is induced in plant explants in response to auxin treatment is closely associated with an extensive genetic reprogramming of the cell transcriptome.
The significant modulation of the gene transcription profiles during SE induction results from the epigenetic factors that fine-tune the gene expression. Goldberg RB, Barker SJ, Perez-Grau L. Regulation of gene expression during plant embryogenesis. Cell. Jan 27; 56 (2)– Graham IA, Smith LM, Leaver CJ, Smith SM.
Developmental regulation of expression of the malate synthase gene. Microspore-derived embryos of Brassica napus cv Reston were used to examine the effects of exogenous (+)-abscisic acid (ABA) and related compounds on the accumulation of very-long-chain monounsaturated fatty acids (VLCMFAs), VLCMFA elongase complex activity, and induction of the 3-ketoacyl-coenzyme A synthase (KCS) gene.
"This book describes how the genome sequence contributes to our understanding of allopolyploidisation and the genome evolution, genetic diversity, complex trait regulation and knowledge-based breeding of this important crop. gene loss, expression divergence, and epigenetic regulation in Brassica napus --Brassica.
Effects of ectopic expression of Brassica genes on Arabidopsis somatic embryogenesis. Given the very low efficiency of B. napus Topas (DH embryogenic line) transformation, the effects of ectopic expression of the four Brassica genes during in vitro embryogenesis .Storage protein gene expression, characteristic of mid- to late embryogenesis, was investigated in microspore embryos of rapeseed (Brassica napus).
These embryos, derived from the immature .An oilseed rape (Brassica napus H) cDNA microarray containing expressed sequence tags (ESTs) was used to analyze the B. napus gene expression changes elicited by oligochitosan.